ISO 16000-3 PDF

Volkis The sampling method gives a time-weighted average TWA sample. Chemical and Textile Sector. Detailed information on cookies and your right of objection can be found in our data privacy statement. It can be used for long-term 1 h to 24 h or short-term 5 min to 60 min sampling of air for formaldehyde. This International Standard describes a sampling and analysis procedure for the determination of formaldehyde HCHO and other carbonyl compounds aldehydes and ketones that involves collection from air onto cartridges coated with 2,4-dinitrophenylhydrazine DNPH and subsequent analysis by high performance liquid chromatography HPLC with detection by ultraviolet absorption.

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Overview Formaldehyde and certain other carbonyl compounds have a high toxic potential. This part of DIN ISO is a test standard for the area of environmental protection and is intended to be used to characterize indoor air with regard to formaldehyde and higher aldehydes and ketones.

The standardized measuring method is important to recognize health-related indoor pollution and may have far-reaching implications as the investigation result can have considerable consequences, for example, with regard to the need of renovation or the success of renovation. In addition to the sphere of private households, internal spaces according to this standard include offices, schools, nursery schools, hospitals, event locations such as theatres, cinemas as well as the cabin interiors of vehicles, amongst others.

The document is directed at representatives of supervisory authorities, testing laboratories, professional associations, planning offices, the furniture industry, the paint industry, the adhesives industry, interior designers and the automotive industry.

Formaldehyde is the simplest carbonyl compound, with one carbon, one oxygen and two hydrogen atoms. In its monomolecular state, it is a colourless, pungent, reactive gas. It is used in the production of urea-formaldehyde resins, adhesives, and insulating foams. Emissions from particle chip board and wall insulation are the major sources of formaldehyde in indoor air.

The method is specific to formaldehyde but, with modification, other carbonyl compounds can be analysed. Formaldehyde or the carbonyl compounds are collected by drawing air through a cartridge containing a substrate silica gel coated with 2,4-dinitrophenylhydrazine DNPH reagent. The principle of the method is based on the specific reaction of a carbonyl group with DNPH in the presence of an acid. During the process stable derivatives hydrazones of lower vapour pressure are formed according to the reaction equation which can be more efficiently retained and easily analysed.

The hydrazones can be separated, identified and quantified with high sensitivity after extraction during subsequent analysis utilizing high performance liquid chromatography HPLC with UV detection or diode array detection. The method has been tested in an exemplary manner for the following 13 aldehydes and ketones: formaldehyde, acetaldehyde, acetone, benzaldehyde, butyraldehyde, capronaldehyde, 2,5-dimethylbenzaldehyde, isovaleraldehyde, propionaldehyde, m-tolualdehyde, o-tolualdehyde, p-tolualdehyde, valeraldehyde.

Other carbonyl compounds that may be emitted into air from solvents, adhesives, cosmetics, and other sources can also be determined using this part of ISO The sampling method gives a time-weighted average TWA sample. It can be used for long-term 1 h to 24 h or short-term 5 min to 60 min sampling of air for formaldehyde. The secretariat of SC 6 and the chairmanship are held by Germany.


DIN ISO 16000



DIN ISO 16000-3



BS ISO 16000-3:2011



ISO 16000-6:2011


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